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Fig. 3 | Cancer Cell International

Fig. 3

From: Long noncoding RNA DLX6-AS1 promotes neuroblastoma progression by regulating miR-107/BDNF pathway

Fig. 3

DLX6-AS1 can bind with miR-107 and knockdown of miR-107 attenuates siDLX6-AS1 inhibited effects on NB cell proliferation, migration and invasion. a The predicted binding sites of miR107 to the DLX6-AS1 sequence. b Luciferase reporter assays were performed for the detection of the luciferase activities of HEK293T cells after transfections. The qRTPCR results of miR-107 expression in c NB tissues and d NB cells. e Spearman’s correlation curve indicated a negative correlation between DLX6-AS1 and miR107 in NB tissues. f The relative miR107 expression in SKNSH and SHSY5Y cells after transfection with siNC, siDLX6-AS1, inhibitor NC or miR107 inhibitor was tested by qRTPCR analysis (24 h). g MTT assay (1–5 days) and h colony formation assay (2 weeks) results showed cell viability and cell proliferation abilities in two NB cells in different groups. Cell migration and invasion capacities in two NB cells with different transfections were determined by i wound healing assay (24 h) and j transwell assays (24 h), respectively. k Flow cytometry analysis (48 h) was conducted to analyze the apoptosis rates in SKNSH and SHSY5Y cells in different group. *p < 0.05, **p < 0.01 and ***p < 0.001

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