Fig. 2

KLF8 knockdown reduces the growth of lung cancer cells in vitro. a–d A549 and H1299 cells were infected with lentivirus carrying shKLF8 or control shRNA and were selected with puromycin. The transduced cells were subjected to analyze the mRNA (a, c) and protein (b, d) level of KLF8. **p < 0.01 vs. shCtrl. n = 3 in each group. e, f A549 and H1299 cells with/without shKLF8 transduction were subjected to proliferation assay (e, g) and colony formation assay (f, h). **p < 0.01 vs. shCtrl. n = 3 in each group. The number of cells was normalized to the control group at day 0 (%) and the percentage of cell number was shown