Fig. 3

KLF8 overexpression promotes the growth of lung cancer cells in vitro. a A549 cells were infected with lentivirus carrying KLF8 or control constructs and the cells were selected with puromycin. The transduced cells were subjected to analyze the protein level of KLF8. b, c A549 cells with/without KLF8 transduction were subjected to proliferation assay (b) and colony formation assay (c). *p < 0.05 vs. Lenti-Ctrl. n = 3 in each group. The number of cells was normalized to the control group at day 0 (%) and the percentage of cell number was shown. d H1299 cells were infected with lentivirus carrying KLF8 or control constructs and the cells were selected with puromycin. e H1299 cells with/without KLF8 transduction were subjected to proliferation assay. *p < 0.05 vs. Lenti-Ctrl. n = 3 in each group. f BEAS-2B cells were infected with lentivirus carrying KLF8 or control constructs and the cells were selected with puromycin. g BEAS-2B cells with/without KLF8 transduction were subjected to proliferation. *p < 0.05 vs. Lenti-Ctrl. n = 3 in each group