Fig. 4

KLF8 regulates cell cycle. a KLF8 effects on cellular apoptosis. A549 cells were infected with indicated lentivirus for 24 h, then cellular apoptosis was analyzed. n = 3 in each group. b KLF8 knockdown effects on cell cycle. A549 cells were infected with indicated lentivirus for 24 h, then the cell cycle was analyzed. ** p < 0.01 vs. shCtrl. n = 3 in each group. c KLF8 overexpression effects on cell cycle. A549 cells were infected with indicated lentivirus for 24 h, then the cell cycle was analyzed. **p < 0.01 and ***p < 0.001 vs. shCtrl. n = 3 in each group. d Effects of KLF8 knockdown on the expression of cell cycle regulators. A549 cells were infected with indicated lentivirus for 24 h, then the mRNA level of cell cycle regulators was analyzed. ***p < 0.001 vs. shCtrl. n = 3 in each group. e Effects of KLF8 overexpression on the expression of cell cycle regulators. A549 cells were infected with indicated lentivirus for 24 h, then the mRNA level of cell cycle regulators was analyzed. **p < 0.01 vs. Ctrl. n = 3 in each group. f Western blot analysis of the effects of KLF8 on P21 expression in A549 cells. The cells were treated as in d and e