Fig. 5

JMJD2A is involved in the regulation of the cell cycle and cell growth by KLF8. a The mRNA level of JMJD2A is overexpressed in human lung cancer cell lines (A549, H1975, H1299, H460, and H520) compared with normal lung epithelial cell line (BEAS-2B). *p < 0.05 and ***p < 0.001. n = 3 in each group. b JMJD2A knockdown in A549 cells. A549 cells were infected with indicated lentivirus for 24 h, then the mRNA and protein levels of JMJD2A were analyzed. *p < 0.05 vs. shCtrl. n = 3 in each group. c, d JMJD2A knockdown blocks KLF8 effects on the expression of P21 and CDK4. A549 cells were infected with indicated lentivirus for 24 h, then the mRNA and protein level of P21 (c) and CDK4 (d) were analyzed. **p < 0.01. n = 3 in each group. e JMJD2A knockdown blocks KLF8 effects on cell cycle. A549 cells were infected with indicated lentivirus for 24 h, then the cell cycle was analyzed. *p < 0.05 vs. Ctrl. f, g A549 cells were infected with indicated lentivirus and selected with puromycin and then the transduced cells were subjected to cell proliferation (f) and colony formation assay (g). *p < 0.05 vs. Ctrl. ^##p < 0.01 vs. Lenti-KLF8. n = 3 in each group. The number of cells was normalized to the control group at day 0 (%) and the percentage of cell number was shown