Fig. 1

KDM3B is required for the growth and granulocytic differentiation of NB4 cells. a, b The mRNA expression levels of KMD3B across various types of human cancers. Data were retrieved from CCLE dataset (a), which contains RNA-seq of 1457 human cancer cell lines, and the TCGA dataset (b). Arrow, leukemia and lymphoma cells. c KDM3B mRNA levels in AML patients and normal counterparts were compared (raw data retrieved from from GSE9476 and GSE7186. d Overall survival of AML patient cohort with regard to KDM3B mRNA levels. e Effects of stable KDM3B-knockdown in APL NB4 cells by Western blot analysis. f Knock-down of KDM3B promotes NB4 cell proliferation. CCK8 assay was performed to determine the cell growth of NB4 cells transfected with scramble control or shKDM3B. g Cell cycle was determined by flow cytometry analysis. The bar chart represented the percentage of cells in G0/G1, S, or G2/M phases respectively. h Flow cytometric analysis of CD11b cell-surface expression in NB4 cells transfected with scramble control or shKDM3B followed by treatment with or without ATRA. Values are derived from three independent experiments, and data were mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001