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Fig. 1 | Cancer Cell International

Fig. 1

From: Long non-coding RNA HOTAIR functions as a competitive endogenous RNA to regulate PRAF2 expression by sponging miR-326 in cutaneous squamous cell carcinoma

Fig. 1

Regulatory effects of HOTAIR on proliferation, apoptosis and cell cycle progression of CSCC cells. a Detection of HOTAIR expressions in CSCC cell lines (A431, SCL-1, HSC-5, and SCC13) and one human keratinocyte cell line (HaCaT) via qRT-PCR. b CCK-8 assay shows the proliferation of A431 transfected with HOTAIR siRNAs and SCL-1 transfected with HOTAIR OE plasmids. c, d EdU assay shows the proliferation of A431 transfected with HOTAIR siRNAs and SCL-1 transfected with HOTAIR OE plasmids. e Determination of cell cycle through BD Biosciences FACSCalibur™ Flow Cytometry. f Detection of cell apoptosis through BD Biosciences FACSCalibur™ Flow Cytometry. g Detection of the expression of Cyclin D1, pro-caspase-3, cleaved-caspase-3 through western blot. Data are presented as mean ± SD. *P < 0.05; ns, no significant difference; siRNA NC, siRNA negative control group; vector, overexpression plasmid vector negative control group

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Cancer Cell International

ISSN: 1475-2867

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