Fig. 1

Expression and prognostic value of CDK5RAP3 in GNEC. a Immunohistochemical staining of CDK5RAP3 expression in GNEC tissues and criteria for immunohistochemistry scoring. Score 0: no staining, Score 1: weak staining, Score 2: moderate staining, Score 3: strong staining. Each section was examined under a high-power field (40×). b Representative image of CDK5RAP3 protein expression in GNEC tissues and adjacent normal gastric tissues analyzed using IHC. c IHC scores of CDK5RAP3 in GNEC tissues and adjacent normal gastric tissues was compared using Student’s t-test. n = 51 (*P < 0.05). d Protein levels of CDK5RAP3 in GNEC tissues and adjacent normal gastric tissues from 8 additional patients measured by western blot analysis. e Kaplan–Meier survival curve of gastric cancer patients with “CDK5RAP3 high” and “CDK5RAP3 low” expression levels (P < 0.05, log-rank test). CD31 staining was used to determine MVD. f Representative images microvessel staining (CD31+) under high-power fields (20×, 40×). g MVD was calculated as the average measurement of 10 random high-power fields (20×). Comparison of MVD in GNEC tissues stratified by different CDK5RAP3 levels. Low CDK5RAP3 tissues: 39 ± 3.75 per field, n = 23 cases; High CDK5RAP3 tissues: 28 ± 1.94 per field, n = 28 cases, P < 0.01. Scale bar, 50 µm