Fig. 2

CDK5RAP3 in GNEC cells indirectly affects endothelial cell migration and tube formation in vitro. ECC10 and ECC12 cells with stable overexpression or knockdown of CDK5RAP3 were created. a CDK5RAP3 expression changes were confirmed using western blot analysis. CDK5RAP3 mRNA changes were confirmed using RT-PCR. b CDK5RAP3 inhibits tumor-induced HUVEC migration as shown by transwell migration assays. Representative images are shown under high-power fields (10×) (c). Quantification of results is presented in d. CDK5RAP3 inhibits tumor-induced HUVEC angiogenesis as shown by tube formation assays. Representative images are shown under high-power fields (10×) (e). Quantification of the results is presented in f. CDK5RAP3 in ECC10 cells indirectly inhibits tumor-induced HUVEC migration as shown by wound healing assay. Representative images are shown under high-power fields (10×) (g). Quantification of results is presented in h. Results show mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar, 200 µm