Fig. 3

Function study of CKS1 in vivo. CKS1-expressing HCT116 cells were injected into the BALB/c nude mice to study the function of CKS1 in tumorigenesis, GFP-expressing HCT116 cells were injected into the BALB/c nude mice as the control group; a western blot analysis was used to confirm CKS1 expression level both in CKS1-expressing HCT116 cells and in GFP-expressing HCT116 cells; b tumors were dissected 42 days after cell injection, the upper group of tumors was developed from CKS1-expressing HCT116 cells, and the lower group of tumors was developed from GFP-expressing HCT116 cells; c tumors weight were measured and statistically analyzed; d qRT-PCR was used to measure the expression level of CKS1 in the tumor tissues dissected after 42 days injection. *Indicates p < 0.05 for three independent experiments analyzed by Student’s t test