Fig. 4

MiR-135a-5p overexpression suppressed tumor cell progression and KLF8 expression in vitro. a Relative expression of miR-135a-5p in different TSCC cell lines was examined using qRT-PCR. b Relative expression of miR-135a-5p was measured in CAL-27 and TCa-8113 cells transfected with miR-135a-5p mimics by qRT-PCR. c The viability of CAL-27 and TCa-8113 cells was measured using MTT assay. d, e The migration and invasion of CAL-27 and TCa-8113 cells was examined using wound healing assay and transwell assay, respectively. f Relative expression of KLF8 mRNA was detected in CAL-27 and TCa-8113 cells using qRT-PCR. g Relative expression of KLF8 protein was measured using western blot in CAL-27 and TCa-8113 cells. *p < 0.05, **p  < 0.01, ***p < 0.001, versus to NC mimics