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Fig. 2 | Cancer Cell International

Fig. 2

From: MicroRNA-346 inhibits the growth of glioma by directly targeting NFIB

Fig. 2

miR-346 overexpression induces cell cycle arrest and inhibits glioma cell growth in vitro. a CCK-8 assay of proliferation of U87 and U251 glioma cell lines transfected with miR-NC or miR-346. b Colony-forming assays of U87 and U251 cells transfected with miR-NC or miR-346. c Representative single or merged images of DAPI- and EDU-stained U87 and U251 cells transfected with miR-NC or miR-346. d Flow cytometric analysis of cell cycle phase of U87 and U251 cells transfected with miR-NC or miR-346. e Western blot analysis of Cyclin E1, Cyclin D1 and CDK4 in U87 and U251 cells 48 h after transfection with miR-NC or miR-346. GAPDH served as a loading control. **P < 0.01, ***P < 0.001

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