Fig. 5

hnRNPK regulates the alternative splicing of CD44 through SRSF1. a Schematic diagram showing the different splicing variants of CD44 (full-length CD44), CD44V6 (only contains variable exon 6), CD44V8-10 (contains variable exons 8–10) CD44V6-10 (contains variable exons 6–10) and CD44S (has no variable exons) isoforms. The arrows indicate the location of specific primer sets designed for qRT-PCR analysis of different IRF3 splicing variants. b Relative expression of CD44 splicing variants in MGC-803 cells after transfected with si_control or si_HNRNPK. c Relative expression of CD44 splicing variants in MGC-803 cells after transfected with si_control or si_SRSF1 (upper), or pCDNA or pCDNA-SRSF1 (lower). d Relative expression of CD44 splicing variants in MGC-803 cells after transfected with after co-transfection with si_hnRNPK and pCDNA-SRSF1 or their negative controls. For all quantitative results, the data are presented as mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *p < 0.05