Fig. 3

PAX8 regulates the expression of the ITGB3 gene. a PAX8, ITGB3 and ITGAV expression levels were measured by qRT-PCR in Primary hFTSEC, SKOV3, KURAMOCHI, OVSAHO and PEA1 48 h after RNAi of PAX8. The values are mean ± SD of three independent experiments in duplicate, normalized by the expression of ABL and expressed as fold change with respect to the siCTR cells, whose value was set at 1.0. p-value was calculated by t-test 0.001 ≤ p ≤ 0.1. b Chromatin immunoprecipitation assay was performed to determine the binding of PAX8 to the 5′-flanking regions of ITGB3. Chromatin was subjected to qPCR analysis using appropriate primers (see “Methods”)