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Fig. 2 | Cancer Cell International

Fig. 2

From: The long noncoding RNA PCGEM1 promotes cell proliferation, migration and invasion via targeting the miR-182/FBXW11 axis in cervical cancer

Fig. 2

PCGEM1 promotes CC proliferation. a HeLa cells who had highest PCGEM1 were transfected with negative control siRNA (siNC) or two different specific siRNAs targeting PCGEM1 (siPCGEM1-1 and siPCGEM1-2). After 48 h, the PCGEM1 levels were determined by qRT-PCR. b SiHa cells who had lowest expression level of PCGEM1 were transfected with pcDNA3.1 (NC) or pcDNA3.1-PCGEM1 (PCGEM1) After 48 h, the PCGEM1 levels were determined by qRT-PCR. c The proliferation of HeLa cells with or without PCGEM1 knockdown was assessed by CCK-8 assay. d The proliferation of SiHa cells with or without PCGEM1 overexpression was assessed by CCK-8 assay. e The capability of clone formation is decreased in the siPCGEM1 group. f The capability of clone formation is increased in the PCGEM1 group. g ANXA5 and PI staining showed increased apoptosis of HeLa cells when PCGEM1 was silenced. h ANXA5 and PI staining showed decreased apoptosis of SiHa cells when PCGEM1 was overexpressed. i Western blot analysis showing the expression levels of caspase-3, PARP, and Bax following PCGEM1 alteration in HeLa and SiHa cells, respectively. j Flow cytometry analysis demonstrated that HeLa cells in S-phase population were significantly decreased when PCGEM1 was silenced. k Flow cytometry analysis demonstrated that SiHa cells in S-phase population were significantly increased when PCGEM1 was overexpressed. l Western blot analysis showed the expression levels of CDK4, CDK6, and cyclinD1 following PCGEM1 alteration in HeLa and SiHa cells, respectively. *p < 0.05, **p < 0.01, ***p < 0.001

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