Fig. 6

AvIR-mediated PIT targeting tumor microenvironment. a Effect on FAP-targeted AvIR-PIT on the clonogenicity of MCF-7 cells co-cultured with CAFs. The clonogenicity was evaluated by soft agar colony formation assay as described in Materials and methods. Schematic representation of the soft agar-culturing is shown in the left panel. The number of MCF-7 colonies was counted on 11 days after FAP-targeted AvIR-PIT treatment. The data are the means ± SEM (n = 3). b Effect on CD105-targeted AvIR-PIT on the capillary-like structure formed by 2H-11 tumor endothelial cells. LIVE/DEAD cell imaging was performed against the 2H-11 tubes after AvIR-mediated PIT with Bio-CD105. Data in the rightmost panel shows the relative fluorescence intensity, i.e. the ratio of total green fluorescence intensity per well after NIR irradiation to that of before the irradiation. The data are the means ± SEM (n = 3)