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Fig. 2 | Cancer Cell International

Fig. 2

From: KCNQ1OT1 aggravates cell proliferation and migration in bladder cancer through modulating miR-145-5p/PCBP2 axis

Fig. 2

KCNQ1OT1 serves as miR-145-5p sponge in BC. a QRT-PCR analysis detected the expression of miRNAs after knockdown of KCNQ1OT1. b QRT-PCR assay assessed miR-145-5p expression in BC tissues and normal tissues. c QRT-PCR assay examined the expression of miR-145-5p in cell lines. d Pearson correlation was conducted to explore the correlation between miR-145-5p and KCNQ1OT1 in BC tissues. e Subcellular fractionation and FISH assays determined the distribution of KCNQ1OT1 in nucleus and cytoplasm. GAPDH/U6 was internal control. f The binding sites between KCNQ1OT1 and miR-145-5p were predicted by Starbase. g Dual luciferase report assay verified the binding relation between KCNQ1OT1 and miR-145-5p. h RNA pull down measured the expression of KCNQ1OT1 pulled down by biotin-labeled miR-145-5p group. i The transfection efficiency of miR-145-5p inhibitor was guaranteed by qRT-PCR. j, k CCK-8 and colony formation assays detected cell proliferation in differently transfected groups. l TUNEL assay examined cell apoptosis under different transfecting conditions. m, n Wound healing and transwell assays measured cell migration and invasion respectively among sh-NC, sh-KCNQ1OT1#1 and sh-KCNQ1OT1#1 + miR-145-5p inhibitor groups. o Western blot assay measured protein expressions among sh-NC, sh-KCNQ1OT1#1 and sh-KCNQ1OT1#1 + miR-145-5p inhibitor groups. GAPDH was internal control. *P < 0.05, **P < 0.01

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