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Fig. 2 | Cancer Cell International

Fig. 2

From: SKA1 promotes malignant phenotype and progression of glioma via multiple signaling pathways

Fig. 2

Suppression of SAK1 attenuates the proliferation ability of glioma cells in vitro. a U87, U251, LN229 and T98 cells were transfected with three shRNA vectors against SKA1, and knockdown efficiency were assessed with Western bolt. β-actin served as a loading control. Error bars represent the mean ± SD for three independent experiments (*P < 0.05). b Cell viability of U87 and U251 cells transfected with PLV-Ctr and shSKA1 was evaluated with CCK8 assay, respectively (*P < 0.05). c After SKA1 knockdown, fewer U87 and U251 cells were in S phase as shown in the EdU (red) assay. Nuclei were stained with DAPI (blue). d Proliferation ability of U U87 and U251 cells transfected with PLV-Ctr and shSKA1 was assessed with colony forming assay. Error bars represent the mean ± SD for three independent experiments (*P < 0.05)

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