Fig. 3

HAND2-AS1 targets miR-330-5p. a Prediction of downstream miRNA of HAND2-AS1. The two circles in the figure represent the results of prediction using RN22 and RAID database respectively, and the middle part represents the intersection of the two groups of data. b Detection of hsa-miR-185-5p, hsa-miR-129-5p, hsa-miR-330-5p, hsa-miR-485-5p and hsa-miR-326 expression in cancer tissues and adjacent tissues. N = 68. c Binding sites of miR-330-5p and HAND2-AS1 (N = 68, *p < 0.05 vs. adjacent tissues). d Correlation between HAND2-AS1 and miR-330-5p using TCGA. The abscissa indicates the expression of miRNA, and the ordinate indicates the expression of lncRNA. Each point represents the position of the expression of miRNA and lncRNA in the coordinates in each sample. e Subcellular localization of HAND2-AS1 (×400 magnification, scale bar was 25 μm). f Detection of miR-330-5p expression in 68 cases of cervical cancer and adjacent tissues by RT-qPCR (N = 68, *p < 0.05 vs. adjacent tissues). g Correlation analysis of HAND2-AS1 and miR-330-5p expression. h Binding of HAND2-AS1 and Ago2 determined by RIP. i HAND2-AS1 expression in HeLa cells transfected with miR-330-5p mimic or miR-330-5p inhibitor detected by RT-qPCR (*p < 0.05 vs. cells transfected with NC mimic; ^#p < 0.05 vs. cells transfected with NC inhibitor). j miR-330-5p expression in HeLa cells transfected with oe-HAND2-AS1 or sh-HAND2-AS1 detected by RT-qPCR (*p < 0.05 vs. cells transfected with NC mimic; ^#p < 0.05 vs. cells transfected with NC inhibitor). The above results were measurement data, and expressed as mean ± standard deviation. Paired t-test was applied for comparison of paired data obeying normal distribution and homogeneity of variance between two groups while unpaired t-test was used for comparisons of unpaired data between two groups. The experiment was conducted three times. miR-330-5p microRNA-330-5p, Ago2 Argonaute2, RIP RNA binding protein immunoprecipitation, TCGA The Cancer Genome Atlas