Fig. 3

Uev1A promotes cell survival under serum starvation conditions through the AKT but not NF-κB pathway in breast cancer cells. a UEV1A overexpressed MDA-MB-231-TR cells (left panel) and MCF7 cells (right panel) were treated with LY294002. After 24 h, the AKT pathway proteins were examined by western blot in the whole-cell extract (WCE) or nuclear fraction (N) in UEV1A overexpressed cells alone (UEV1A) or treated with 10 μM LY294002 (1A + LY), or vector only (CK). The expression levels of ectopic Uev1A were monitored by an anti-HA antibody. b, c Growth curve of control (CK) and UEV1A-overexpressed MDA-MB-231-TR (b) and MCF7 (c) cells under serum-deprived conditions. Experimental conditions were as described in Fig. 2c except that some cells were treated with 10 μM LY294002 (LY+). d, e Effects of UEV1A overexpression on the expression of selected FOXO1 target genes. Transcript levels of putative FOXO1 target genes in MDA-MB-231-TR (d) or MCF7 (e) cells overexpressing UEV1A as determined by qRT-PCR. f, g Growth curve of control (CK) and UEV1A-overexpressed MDA-MB-231-TR (f) and MCF7 (g) cells under serum-deprived conditions. Experimental conditions were as described in Fig. 2c except that some cells were treated with 40 μM Bay11-7082 (Bay+). Each sample was measured in triplicate and repeated at least 2 times. *P < 0.05; **P < 0.01