Fig. 5
From: Blocking AMPK/ULK1-dependent autophagy promoted apoptosis and suppressed colon cancer growth
The antitumor effects of NVP-BEZ235 and CQ in vivo. a–c Nude mice were injected s.c. with 1 × 106 HCT116 cells. Once the tumor was measurable, mice were treated daily with 40 mg/kg NVP-BEZ235 by oral gavage, or CQ at 25 mg/kg by i.p. injection, or their combination for 15 consecutive days. a Representative tumors at the end of the experiment. b Tumors weight. c Tumors volume at indicated time points after treatment was calculated. Data represent the mean ± S.D of four independent experiments. d p-AMPK, p-ULK1, LC3-II and cleaved-caspase3 expression were analyzed by western blotting in representative tumors. Similar results were obtained from three independent experiments