Fig. 3

Circ_LARP4 up-regulates its host gene LARP4 after transcription. a, b Subcellular fractionation assay and fluorescence in situ hybridization (FISH) were performed to determine that circ_LARP4 was located in cytoplasm. c The expression and protein level of LARP4 after increasing circ_LARP4 were respectively determined by qRT-PCR analysis and western blot assays. d Luciferase reporter assay was used to test the luciferase activity of LARP4 promoter region. e qRT-PCR analysis and western blot assays were applied to analyze the mRNA and protein expression of LARP4 in different groups. f The proliferation ability of transfected cells was measured via colony formation. g Cell invasion capability in different groups was evaluated via transwell. h Western blot analysis of migration-related proteins was conducted. Results were revealed as the mean ± SD. **P < 0.01