Fig. 8
AURKA knockdown induced cell apoptosis and cell growth inhibition by repressing the activity of Akt/Stat3 pathway (a) 2.0 × 105 of IMR32 cells were seed into 6-well plate. The next day, cells were transfected with AURKA siRNAs, siAURKA-1, si-AURKA-2, or siRNA CTRL. At 48 h after transfection, cells were harvested and total protein were extracted. Western blotting was performed for AURKA expression. b 2.0 × 105 of IMR32 cells were seed into 6-well plate. The next day, cells were transfected with AURKA siRNA-1. At 48 h after transfection, cells were harvested and cell apoptosis and cell cycle analysis (c) were was performed by flow cytometry. d 5 × 103 of IMR32 cells were seed into 96-well plate. The next day, cells were transfected with AURKA siRNA-1. At d0, d1,d2,d3,d4,d5,d6 time points, MTT assay was adopted for cell vialility test. Each sample was analyzed by triplicates. Error bars correspond to the averages ± S.D. e 2.0 × 105 of IMR32 cells were seed into 6-well plate. The next day, cells were transfected with AURKA siRNA-1. At 48 h after transfection, cells were harvested and western blot was performed for expression of AURKA, pAURKA, Akt, p-Akt, STAT3, p-STAT3, JAK2, and p-JAK2