Fig. 3

LINC00908 competitively binds to miR-483-5p in vitro. a The binding of LINC00908 to miR-483-5p analyzed by DIANA Tools (http://diana.imis.athena-innovation.gr/DianaTools/index.php?r=site/page&vie=software) and lncRNASNP2 database (http://bioinfo.life.hust.edu.cn/lncRNASNP#!/). The two circles in the figure respectively represent the predicted results from the lncRNASNP2 database and the DIANA database, and the middle part represents the intersecting miRNAs predicted from the two databases. b The relationship between LINC00908 and miR-483-5p verified by dual-luciferase reporter assay. *p < 0.05 vs. cells upon mimic NC treatment, c LINC00908 binding to miR-483-5p analyzed by RNA pull-down assay. *p < 0.05 vs. cells upon the Bio-probe NC treatment. d The binding relationship between LINC00908 and AGO2 detected by RIP. *p < 0.05 vs. cells upon the IgG treatment. The data were measurement data and expressed as the mean ± standard deviation. Unpaired t-test was used for data between two groups, while one-way ANOVA was used for data among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated three times