Fig. 8
From: UBQLN4 promotes progression of HCC via activating wnt-β-catenin pathway and is regulated by miR-370
Overexpression of UBQLN4 could reverse tumor suppressive effect of miR-370 in HCC cells. a UBQLN4 expression under condition of miR-370 mimics, miR-370 mimics and UBQLN4 or negative control. b Cell viability of Hep-G2 and SMMC-7721 cells under treatment of miR-370 mimics, miR-370 mimics and UBQLN4 and negative control in 24 h, 48 h and 72 h. c, d Tumor formation ability and invasion ability of Hep-G2 and SMMC-7721 cells under treatment of miR-370 mimics, miR-370 mimics and UBQLN4 and negative control. e Dual luciferase assay demonstrating the effect on TOP/FOP reporter activity in Hep-G2 and SMMC-7721 cells transfected with miR-370 mimics, miR-370 mimics and UBQLN4 and negative control. Results were normalized to a Renilla transfection control. f Expression change of β-catenin, CyclinD1 and Axin2 in HCC cells under treatment of miR-370 mimics, miR-370 mimics and UBQLN4 and negative control. *p < 0.05, **p < 0.01