Fig. 4

POU3F2, a downstream target of miR-490-3p, was over expressed in HCC patients. a POU3F2 was predicted as a target gene of miR-490-3p. b miR-490-3p mimic transfection reduced luciferase activity in WT-POU3F2 group, but had no effect on MUT-POU3F2 group, **P < 0.01. c POU3F2 was up-regulated in HCC patients, P < 0.0001. d High expression of POU3F2 in HCC patients was related with unfavorable prognosis. e The clinical relevance levels of BCYRN1^high/miR-490-3p^low/POU3F2^high in patients with HCC were presented, P < 0.0001. f The clinical relevance levels of BCYRN1^low/miR-490-3p^high/POU3F2^low in patients with HCC were exhibited, P = 0.87. g Upregulation of BCYRN1 elevated the luciferase activity of WT-POU3F2, and this phenomenon was inhibited after miR-490-3p overexpression, **P < 0.01 vs. control, ^##P < 0.01 vs. BCYRN1-OE. h Knockdown of BCYRN1 reduced the luciferase activity of WT-POU3F2, and silencing of miR-490-3p attenuated this trend, **P < 0.01 vs. control, ^##P < 0.01 vs. si-BCYRN1