Fig. 4
From: Adaptor protein LNK promotes anaplastic thyroid carcinoma cell growth via 14-3-3 ε/γ binding
LNK interacts with 14-3-3ε/γ (YWHAE/YWHAG) in ATC cells. a Potential LNK binding partners identified by a FLAG pull-down assay combined with LC–MS. b Co-IP assays confirmed LNK binding to 14-3-3ε/γ (YWHAE/YWHAG) in ATC cells. 14-3-3 proteins were tagged with myc, and LNK was tagged with FLAG. c Real-time PCR showed that the mRNA expression levels of 14-3-3ε and 14-3-3γ were increased in LNK-overexpressing ARO cells. d The protein expression level of 14-3-3 in paraffin-embedded ATC and PTC tissue specimens as measured by IHC. +++: high expression, +: low expression. Original magnification, ×40