Fig. 2

Knockdown of circ_0008035 repressed cell proliferation and induced cell apoptosis and ferroptosis in GC cells. a, b Si-NC or si-circ_0008035 was transfected into HGC-27 and AGS cells and then circ_0008035 expression was examined by qRT-PCR. c, d Cell proliferation in HGC-27 and AGS cells transfected with si-NC or si-circ_0008035 was evaluated by MTT assay. e, f The protein levels of cyclin D1 and PCNA in HGC-27 and AGS cells transfected with si-NC or si-circ_0008035 were determined by western blot assay. g, h Cell apoptosis in HGC-27 and AGS cells transfected with si-NC or si-circ_0008035 was analyzed by flow cytometry analysis. i–l HGC-27 and AGS cells were treated with erastin (10.0 μM)/RSL3 (2.0 μM), erastin (10.0 μM)/RSL3 (2.0 μM) plus ferrostain-1 (2.0 μM), erastin (10.0 μM)/RSL3 (2.0 μM) plus ZVAD-FMK (10.0 μM) or erastin (10.0 μM)/RSL3 (2.0 μM) plus necrosulfonamide (0.5 μM) for 48 h and then cell death was evaluated by MTT assay. m, n HGC-27 and AGS cells were transfected with si-NC or si-circ_0008035 and treated with erastin (10.0 μM) or RSL3 (2.0 μM), and then cell death was evaluated by MTT assay. *P < 0.05