Fig. 3

Circ_0008035 knockdown promoted iron accumulation, lipid peroxidation and suppressed mitochondrial membrane potential in GC cells. HGC-27 and AGS cells were transfected with si-NC and si-circ_0008035 and then treated with or without erastin or RSL3 for 48 h. a, b Total iron level, c, d Fe²⁺ accumulation, e, f MDA level, g, h lipid ROS level, i, j mitochondrial superoxide level and k, l mitochondrial membrane potential in HGC-27 and AGS cells were investigated by specific kits. *P < 0.05