Fig. 4

Circ_0008035 directly interacted with miR-599 and negatively regulated miR-599 expression in GC cells. a The predicted binding sites between circ_0008035 and miR-599 were shown. b, c Dual-luciferase reporter assay was performed to determine the luciferase activity in HGC-27 and AGS cells co-transfected with circ_0008035 WT or circ_0008035 MUT and miR-599 or miR-NC. d, e The expression of miR-599 in GC tissues, cells (HGC-27 and AGS) and corresponding normal tissues and cells (GES-1) was measured by qRT-PCR. f, g The expression of miR-599 in HGC-27 and AGS cells transfected with si-NC or si-circ_0008035 was examined with qRT-PCR. *P < 0.05