Fig. 8

The influences of circ_0008035 knockdown on cell proliferation, apoptosis and ferroptosis were rescued by EIF4A1 in GC cells. HGC-27 and AGS cells were transfected with si-NC, si-circ_0008035, si-circ_0008035 + vector or si-circ_0008035 + EIF4A1. a, b The level of EIF4A1 in HGC-27 and AGS cells was measured by western blot assay. c, d The proliferation of HGC-27 and AGS cells was assessed by MTT assay. e, f The protein levels of cyclin D1 and PCNA in HGC-27 and AGS cells were detected using western blot assay. g, h The apoptosis of HGC-27 and AGS cells was evaluated by flow cytometry analysis. i, j Transfected HGC-27 and AGS cells were treated with erastin or RSL3 for 48 h and then cell death was assessed by MTT assay. *P < 0.05