Fig. 6

Up-regulation of NEK2 reversed the miR-329-3p-induced repressed effects on the glycolysis and radioresistance of glioma cells. a–g U251 and LN229 cells were transfected with miR-NC, miR-329-3p, miR-329-3p + pcDNA-NC or miR-329-3p + pcDNA-NEK2. a, b MTT assay for the viability of transfected glioma cells. c–e The glucose consumption, lactate production and ATP level in transfected U251 and LN229 cells. f, g Western blot assay for the levels of HIF-1α, HK2 and LDH-A in transfected U251 and LN229 cells. h, i Colony formation assay for the survival rate of U251 and LN229 cells transfected with miR-NC, miR-329-3p, miR-329-3p + pcDNA-NC or miR-329-3p + pcDNA-NEK2 under X-ray radiation treatment (0 Gy, 2 Gy, 4 Gy, 6 Gy and 8 Gy). j, k Colony formation assay for the colony formation rate of U251 and LN229 cells transfected with miR-NC, miR-329-3p, miR-329-3p + pcDNA-NC or miR-329-3p + pcDNA-NEK2 treated with 6 Gy of X-rays or not. *P < 0.05