Co-prevalence of human Papillomaviruses (HPV) and Epstein–Barr virus (EBV) in healthy blood donors from diverse nationalities in Qatar

Table 1 The specific primer sets for HPV used for polymerase chain reaction (PCR) amplification

HPV type	Forward primer (5′-3′)	Reverse primer (5′-3′)	Ta (°C)
E6/E7 region
16	ATGCATGGAGATACACCTACATTGCAT	GTTTCTGAGAACAGATGGGGCACAC	61
18	GCTTTGAGGATCCAACACGG	TGCAGCACGAATGGCACTGG	61
31	GGGCTCATTTGGAATCGTGTG	AACCATTGCATCCCGTCCCC	61
33	TGAGGATGAAGGCTTGGACC	TGACACATAAACGAACTGTG	61
35	CTATTGACGGTCCAGCT	TACACACAGACGTAGTGTCG	61
45	CCCACGCGAACCACAG	TCTAAGGTCCTCTGCCGAGC	46.3
51	TACGTGTTACAGAATTGAAG	AACCAGGCTTAGTTCGCCCATT	46.3
52	GCAGAACAAGCCACAAGCAA	TAGAGTACGAAGGTCCGTCG	60
58	CGAGGATGAAATAGGCTTGG	ACACAAACGAACCGTGGTGC	40.5
L1 region
GP5/GP6	TTTGTTACTGTGGTAGATACTAC	GAAAAATAAACTGTAAATCATATT	50.5
6	TGTCCCATCTGCGCACCGAAGAC	CGTACACTGTTTGTGGGCGCTTC	42.5
11	AGTTCCGTAGATGCCAAGGGCA	TGCCTCAGGTGAGGCCCAATGC	42.5
26	TGGTATACAACGAGTGTCAGCTCC	GGGGCAATGATGGCCATGTCG	40.5
39	TGTGCAGTACCAGTGACGGATCG	ATTTTTGGCGTTGTGACTCTGTG	40.5
53	TTGTTCAGTGTACGGGGCTAGC	GTGACGCCATTGCAGTTATCGCCT	43.3
56	CTGGGCACTAGGTCAAAGCCTGCT	CAACCACGCGTAAAAGCACTCAT	53.8
59	AGACACCGTTACATGAGCTGCT	TCATTCTCGGAGTCGGAGTCAG	43.3
66	TGCGGTAGTATCCTTGGGCAGTG	TACAATAAGGGCCACACGCCAA	46.3
68	GTCAAAAAGACGCCCCTGCACCTA	CACACCTTAGGGTAGGGCTACAA	48.8
73	GGGGTGGGCAAAGGTAGGTAGC	ACAATCCAGGGGCCTCTGGTCCGA	48.8
82	TGTCCGTGGACACCTGCGACCA	GTAGTTAAAGGTGATGTGGCAACC	48.8

ISSN: 1475-2867