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Fig. 2 | Cancer Cell International

Fig. 2

From: Novel monoclonal antibodies against thymidine kinase 1 and their potential use for the immunotargeting of lung, breast and colon cancer cells

Fig. 2

Western blot analysis of the generated antibodies for all 6 epitopes. The antibodies capacity to detect human TK1 was tested. a An amount of 0.5 μg of recombinant human TK1 was loaded in an SDS-PAGE and transferred to nitrocellulose membranes. b An amount of 20 μg of serum from a stage IV lung cancer patient was loaded in each lane. The membranes were probed with the custom TK1 antibodies and compared with a commercial TK1 antibody. For serum a transferring antibody was included as a serum loading control and to test serum integrity

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Cancer Cell International

ISSN: 1475-2867

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