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Table 3 Sensitivity of the TK1 antibodies

From: Novel monoclonal antibodies against thymidine kinase 1 and their potential use for the immunotargeting of lung, breast and colon cancer cells

Clone

ID

Isotype

Host

Mean

SD±

Inter CV

Intra CV

LOD pg/ml

LOD

%

%

4G10

1-1

IgG2a, κ

Ms

146.40

33.96

16.4

5.72

*10E8

1-2

IgG1, κ

Ms

44.67

4.33

9.69

11.40

*7H2

1-7

IgG1, κ

Ms

19.79

0.55

2.78

9.71

7D1

1-9

IgG1, κ

Ms

94.03

12.86

13.68

8.41

*1B12

2-1

IgG2a, κ

Ms

38.76

4.84

12.5

4.57

3G7

2-6

IgG2a, κ

Ms

125.96

21.0

16.67

1.09

10H2

2-15

IgG2a, κ

Ms

56.50

15.96

20.3

8.2

*8G2

2-17

IgG2b, λ

Ms

72.87

4.83

1.62

8.38

*9C10

2-19

IgG2a, κ

Ms

11.01

0.67

6.1

4.0

*3B4

3-5

IgG2b, κ

Ms

66.90

3.5

5.33

0.45

6A11

3-6

IgG1, κ

Ms

NA

NA

NA

NA

8F12

3-10

IgG1, κ

Ms

101.00

16

15.84

2.5

5F7G11

4-2

IgG2a, λ

Rat

1376.58

153.42

11.14

0.71

*2E8

5-1

IgG2b, λ

Ms

33.10

0.50

1.52

1.5

9A9

5-10

IgG2b, κ

Ms

12,006.28

244.36

2.04

1.76

*3B2E11

6-1

IgG2a, λ

Rat

10.73

1.85

17.32

6.58

1D3G4

6-2

IgG2a, λ

Rat

NA

NA

NA

NA

EPR3194

Abcam

Unknown

Rb

16.48

1.045

6.33

10.2

91651

  1. The LOD values are expressed as mean values from two independent immunoassays. Precision and uncertainty are reflected in the coefficient of variance between assays. The most sensitive antibodies are marked with stars