Fig. 2

Comparison of KIF15 expression levels in different GC cell lines and knockdown efficiency of KIF15 in two GC cell lines. a The mRNA expression level of KIF15 was higher in GC cell lines compared with normal gastric cell line. b The results of bright and dark microscopy fields showed that the transfection efficiency of sh-KIF15 in two GC cell lines was significant. c RNA was extracted from shKIF15-AGS, shKIF15-SGC-7901 cells, and corresponding control cells. qRT-PCR proved that KIF15 expression level was significantly decreased in sh-KIF15 group in AGS and SGC-7901 cells compared with sh-Ctrl group (P < 0.001). d WB results also proved that the protein expression level of KIF15 was downregulated in sh-KIF15 group compared with sh-Ctrl group in GC cell lines AGS and SGC-7901. GAPDH was used as an internal control