Table 1 The sequences were used in this study
From: MicroRNA-196b-5p promotes malignant progression of colorectal cancer by targeting ING5
Name | Sequences (5′–3′) |
|---|---|
miR-196b-5p | Stem-loop RT primer: GCGCGTGAGCAGGCTGGAGAAATTAACCACGCGCCCCAAC |
F: GCGTAGGTAGTTTCCTG | |
R: GAGCAGGCTGGAGAA | |
miR-196a-1 | Stem-loop RT primer: GCGCGTGAGCAGGCTGGAGAAATTAACCACGCGCTCGGGTG |
F: CGCAACAACATTAAACC | |
R: GAGCAGGCTGGAGAA | |
miR-196a-2 | Stem-loop RT primer: GCGCGTGAGCAGGCTGGAGAAATTAACCACGCGCCTCAGG |
F: CGGCAACAAGAAACTG | |
R: GAGCAGGCTGGAGAA | |
ING5 | F: GCACAAAGGAGGGTCTGA |
R: TGGGTTTCGTGGTAAGGT | |
U6 | F: GCTTCGGCAGCACATATACT |
R: GAGCAGGCTGGAGAA | |
β-actin | F: CTTAGTTGCGTTACACCCTTTCTTG |
R: CTGTCACCTTCACCGTTCCAGTTT | |
miR-196b-5p mimics | TAGGTAGTTTCCTGTTGTTGGG |
miR-196b-5p inhibitor | CCCAACAACAGGAAACTACCTA |
miR-196b-5p NC mimics | TTCTCCGAACGTGTCACGT |
miR-196b-5p NC inhibitor | TTCTCCGAACGTGTCACGT |
ING5 shRNA | F: CAAGGAATACAGTGACGACAA |
R: TTGTCGTCACTGTATTCCTTG |