Fig. 1

LINC01419 acts as an oncogene in OS. a LINC01419 expression was assessed in OS cell lines (143B, U2OS and Saos-2) and human normal osteoblast (hFOB1.19) by using qRT-PCR analysis. b Silencing efficiency of LINC01419 was evaluated in U2OS and Saos-2 cells by qRT-PCR. c–d. Colony formation assay and EdU assay (scale bar = 200 μm) were conducted to evaluate the proliferation of U2OS and Saos-2 cells transfected with sh-LINC01419#1/2 or sh-NC. e–f. U2OS and Saos-2 cell apoptosis was examined by JC-1 assay (scale bar = 200 μm) and TUNEL assay (scale bar = 200 μm) in sh-LINC01419 group or sh-NC group. g–h. U2OS and Saos-2 cells migration and invasion were illustrated by transwell assay (scale bar = 200 μm) with the transfection sh-LINC01419 or sh-NC. i–j. IF (scale bar = 50 μm) and western blot assay were conducted to investigate the EMT process of sh-LINC01419 or sh-NC transfected U2OS and Saos-2 cells. ^*P < 0.05, ^**P < 0.01