Fig. 1

BSN-AS2 was transcriptionally activated by E2F1 in spinal OS. a The expression of BSN-AS2 in spinal OS tissues and adjacent-normal tissues was measured by qRT-PCR. b qRT-PCR revealed BSN-AS2 expression in spinal OS cells and hFOB cells. c JASPAR database presented the binding motif of E2F1 and the binding area of E2F1 on BSN-AS2 promoter. d The schematic diagram about role of E2F1 in BSN-AS2 transcription. e ChIP assay illustrated the binding facts between E2F1and BSN-AS2 promoter in part 2 (P2). f–g qRT-PCR and western blot assays detected the mRNA and protein expressions of E2F1. h qRT-PCR assessed the expression of BSN-AS2 in response to E2F1 upregulation or downregulation. i Luciferase reporter assays demonstrated the effects of E2F1 expression modulation on BSN-AS2 transcription. j Luciferase reporter assays verified binding facts between E2F1 and BSN-AS2 promoter in P2. k The expression of E2F1 in spinal OS tissues and adjacent-normal tissues was measured by qRT-PCR (left); Pearson correlation analysis illustrated the correlation between E2F1 and BSN-AS2 (right). ^*P < 0.05, ^**P < 0.01