Fig. 4
HM inhibits Bcl2 family proteins and induces cytochrome c in CRC cells. The effect of HM on Bcl2 family proteins was investigated using Western blot technology. Untreated and HM-treated HT29 (a) and SW620 (b) cell lysates were immunoblotted with the antibodies directed against the indicated proteins. Bar graphs showing the Bcl2 protein expression relative to the basal expression detected in control cells given an arbitrary value of 1.0 using β-actin as the loading control. All data present the mean + SD of three independent experiments. (*), (**), and (***) signify a statistically significant difference (P < 0.05, P < 0.01, and P < 0.001) compared with the control