Fig. 5

miR-135b suppressed JADE-1 expression specifically. a The binding site of miR-135b to JADE-1 (aliasing PHF-17) 3′UTR was analyzed by Targetscan. b dual-luciferase reporter gene assay was used to determine the targeting relationship between miR-135b and JADE-1 (*p < 0.05 vs. the agomir-NC group); c RT-qPCR was applied to measure JADE-1 mRNA expression in PCSCs of each group. d western blot analysis was used to examine the expression of JADE-1 protein in PCSCs of each group. *p < 0.05 vs. The mimic-NC or antagomir-NC group, ^#p < 0.05 vs. the agomir-NC group. The measurement data were expressed as mean ± standard deviation. One-way ANOVA was applied for comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated 3 times