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Table 1 RT-qPCR primer sequences

From: Silencing of microRNA-135b inhibits invasion, migration, and stemness of CD24+CD44+ pancreatic cancer stem cells through JADE-1-dependent AKT/mTOR pathway

miRNA/gene

Primer sequence

miR-135b

F: 5′-CTGTGGCCTATGGCTTTTCAT-3′

R: 5′-GCTCGCCCCTCACTGTAG-3′

U6

F: 5′-CGCTTCGGCAGCACATATAC-3′

R: 5′-AAATATGGAACGCTTCACGA-3′

JADE-1

F: 5′- AGGGATTAAAGTGCTCCCCC-3′

R: 5′-TGGCGTTTGGCTTGTTATGG-3′

GAPDH

F: 5′-AATGGGCAGCCGTTAGGAAA-3′

R: 5′-GCGCCCAATACGACCAAATC-3′

CD24

F: 5′-CCCCACCTTGCCTGCG-3′

R: 5′-AAATCTGCGTGGGTAGGAGC-3′

CD44

F: 5′-TTACAGCCTCAGCAGAGCAC-3′

R: 5′-TGACCTAAGACGGAGGGAGG-3′

ESA

F: 5′-TGGTTTCAGGGGGCTGTTGT-3′

R: 5′-TCGGCCGTCTCTACGTCCTC-3′

Sox-2

F: 5′-GCCTGGGCGCCGAGTGGA-3′

R: 5′-GGGCGAGCCGTTCATGTAGGTCTG-3′

Oct-4

F: 5′-GCTCGAGAAGGATGTGGTC-3′

R: 5′-ATCCTCTCGTTGTGCATAGTCG-3′

Nanog

F: 5′-GCTGAGATGCCTCACACGGAG-3′

R: 5′-TCTGTTTCTTGACTGGGACCTTGTC-3′

ALDH1

F: 5′-GCACGCCAGACTTACCTGTC-3′

R: 5′-CCTCCTCAGTTGCAGGATTAAAG-3′

Slug

F: 5′-CGAACTGGACACACATACAGTG-3′

R: 5′-CTGAGGATCTCTGGTTGTGGT-3′

  1. RT-qPCR reverse transcription quantitative polymerase chain reaction, microRNA-135b miR-135b, JADE-1 jade family PHD finger 1, GAPDH glyceraldehyde 3-phosphate dehydrogenase, ESA electrical stimulation for analgesia, F forward primer, R reverse primer, Sox-2 SRY related HMG box-2, Oct-4 Octamer-4, ALDH1 aldehyde dehydrogenase isoform 1