Fig. 5

ZIC3 could be the target of miR-24-1. a The RNA quantification of the HCC70 cells transfected with miR-24-1 and miR-NC according to the RT-qPCR. **P value< 0.01. b The schematic diagram listed the conjectural miR-24-1 binding sites within the 3′UTR of ZIC3 as well as the sequences of wild-type and mutant 3′UTR of ZIC3. The luciferase activity in HCC70 cells was detected by using luciferase reporter gene assays (**P value < 0.01). c The scatter plot presented the relative RNA levels of the 3′UTR of ZIC3 and GAPDH that were quantified by the RT-qPCR after transfection in the HCC70 cells. (**P value< 0.01). d, e The expression level of mRNA and protein of ZIC3 in melanoma cells with miR-24-1 inhibitor and NC were presented. f Cell proliferation according to the CCK-8 assay (*P value< 0.05). g HCC70 cell invasive capacity based on the transwell assay (**P value < 0.01). The validation of IGBP1-AS1/miR-24-1/ZIC3 axis in vivo. h The bioluminescent imaging of the mice was collected by IVIS bioluminescence imaging system after transplantation of luciferase expressing HCC70 cells transfected with NC, Lv-IGBP1-AS1, miR-24-1 mimic, Lv-IGBP1-AS1 + Si-ZIC3, Lv-ZIC3 + miR-24-1 mimic