Table 2 The primer design of eIF3 subunits and GAPDH
From: Roles of eIF3m in the tumorigenesis of triple negative breast cancer
Genes | Direction | Sequence (5′ to 3′) | Length (bp) |
|---|---|---|---|
eIF3a | Forward | GAGCGATCATCCTGGCGTAA | 332 |
Reverse | GGTCTTGAGTCATCACCCCG | ||
eIF3b | Forward | GCACTGTGGGGACGGAC | 261 |
Reverse | TGGAAGCGGGTGCCTTAAAT | ||
eIF3c | Forward | GGGCTCAGCTGGTTGGTATT | 207 |
Reverse | CCGAACCGGTGGTGAAAAAC | ||
eIF3d | Forward | TCGGCTAGGAAAGGTTGCAG | 217 |
Reverse | TTTGTCTCTGCGGAGGTTCC | ||
eIF3e | Forward | TGCAAGACTGGATGCCAAGA | 185 |
Reverse | TTGCCCAGTTAGGAGCCTCT | ||
eIF3f | Forward | CTGCACCCAGTCATTTTGGC | 297 |
Reverse | TCGGCTGTAGTACTCGTGGA | ||
eIF3g | Forward | GAGACCCGGAAGGCTTCAAA | 205 |
Reverse | ACACGATCTTCTGGCCCTTG | ||
eIF3h | Forward | AAAACAAGCCCTGACCGGAA | 187 |
Reverse | TCATTTGGGAGCAGCGAAGT | ||
eIF3i | Forward | CGGGATGAAGCCGATCCTAC | 316 |
Reverse | CCCAAAGTCAAAACCGCAGG | ||
eIF3j | Forward | GGACGTCAAGGATAACTGGGA | 324 |
Reverse | TCTCTTGAAGATGGGTTCATAGCA | ||
eIF3k | Forward | TGCTCAAGGGTATCGACAGG | 267 |
Reverse | AAATCTGTCGGATTGGCCGT | ||
eIF3l | Forward | AAGCCATTGCTCCACAGGTT | 273 |
Reverse | TCTTGGCAGTCTTACAGCGG | ||
eIF3m | Forward | GACATCAGTGAAGAAGATCAGGC | 150 |
Reverse | ATCATCCTCCTTCAGACACACA | ||
GAPDH | Forward Reverse | GAAGGTGAAGGTCGGAGT | 226 |
GAAGATGGTGATGGGATTTC |