Fig. 4

MIR4435-2HG targeted miR-128-3p to regulate its expression. a The expression of miR-128-3p in OC tissues (n = 42) and adjacent noncancerous tissues (n = 42) was measured by qRT-PCR. b The correlation between MIR4435-2HG expression and miR-128-3p expression in OC tissues was analyzed by Spearman’s correlation analysis. c The expression of miR-128-3p in ISOE80, SKOV3, and OVCAR3 cells was determined by qRT-PCR. d Sequences of miR-128-3p with the putative binding sites of MIR4435-2HG were predicted by starBase v2.0. e The relationship between miR-128-3p and MIR4435-2HG was verified using dual-luciferase reporter assay. f–h The interaction between MIR4435-2HG and miR-128-3p in SKOV3 and OVCAR3 cells was further verified by RIP assay and pull-down assay. i, j qRT-PCR was used to manifest the effect of MIR4435-2HG upregulation on the expression levels of MIR4435-2HG and miR-128-3p in SKOV3 and OVCAR3 cells. k The expression of miR-128-3p in SKOV3 and OVCAR3 cells transfected with si-MIR4435-2HG was examined by qRT-PCR. *P < 0.05