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Fig. 7 | Cancer Cell International

Fig. 7

From: LncRNA MIR4435-2HG triggers ovarian cancer progression by regulating miR-128-3p/CKD14 axis

Fig. 7

CDK14 is a target of miR-128-3p. ac The expression of CDK14 in OC tissues (n = 42) and adjacent normal tissues (n = 42) was detected by qRT-PCR and western blot. d The correlation between CDK14 and miR-128-3p expression was analyzed by Spearman’s correlation analysis. e, f The expression level of CDK14 in ISOE80, SKOV3, and OVCAR3 cells was examined by qRT-PCR and western blot, respectively. g The specific binding regions between CDK14 and miR-128-3p were obtained from the online software starbase. hk The relationship between CDK14 and miR-128-3p was confirmed by dual-luciferase reporter assay, RIP assay and RNA pull-down assay. l The expression of miR-128-3p in SKOV3 and OVCAR3 cells transfected with miR-128-3p inhibitor was analyzed by qRT-PCR. mp The expression of CDK14 at mRNA and protein levels in SKOV3 and OVCAR3 cells transfected with miR-128-3p inhibitor or mimics was measured by qRT-PCR and western blot. *P < 0.05

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