Fig. 6

Silencing of TSC1 alleviated miR-27a-3p knockdown-mediated effects on the cellular behaviors of MM cells. NCI-H929 and MM1.S cells were transfected with anti-NC, anti-miR-27a-3p, anti-miR-27a-3p + scramble or anti-miR-27a-3p + si-TSC1. a The relative expression of TSC1 protein in transfected cells was detected by western blot assay. b Cell viability of transfected cells was examined via CCK-8 assay. c The colony formation ability of transfected cells was evaluated by colony formation assay. d, e The apoptosis rate and cell cycle distribution were detected through flow cytometry assay. f, g The migration and invasion abilities of transfected cells were measured via transwell assay. h, i The protein levels of Cleaved caspase 3/total caspase 3, Cleaved caspase 1, γ-H2AX, Cyclin D1, p21, Ki-67, MMP9, MMP7 and MMP10 were analyzed by western blot analysis. ^*P < 0.05