Fig. 4

MiR-506-3p could be sponged by circ_100565. a The expression changes of 11 targeted miRNAs in A549 cells transfected with sh-circ_100565 or sh-NC were detected by qRT-PCR. b The sequences of circ_100565 contained the miR-506-3p binding sites or mutant binding sites were shown. c, d Dual-luciferase reporter assay was used to detect the interaction between miR-506-3p and circ_100565 in A549 and H1299 cells. e RIP assay was performed to determine the enrichment of circ_100565 and miR-506-3p in anti-ago2 or anti-IgG. f The effect of sh-circ_100565#2 on the expression of miR-506-3p in A549 and H1299 cells was detected by qRT-PCR. g The expression of miR-506-3p in NSCLC cell lines (Calu-3, Calu-6, A549 and H1299) and HBE1 cells was determined by qRT-PCR. h QRT-PCR was performed to assess the expression of circ_100565 in NSCLC tissues (NSCLC) and adjacent normal tissues (Normal). i The correlation between miR-506-3p and circ_100565 expression was measured by Pearson correlation analysis. *P < 0.05, **P < 0.01, ***P < 0.001