Fig. 3

FLVCR1-AS1 activates Wnt/β-catenin pathway. a Western blot was performed to measure expression of relevant proteins engaging in common tumor signaling pathway after knockdown of FLVCR1-AS1. b FOP/TOP assay was used to verify the activity of Wnt/β-catenin Pathway. c MTT was performed to assess cell proliferation after adding agonist of Wnt/β-catenin pathway. d TUNEL was performed to investigate apoptosis after adding agonist of Wnt/β-catenin pathway. e, f Transwell assays were conducted to determine cell migration and invasion abilities respectively after adding agonist of Wnt/β-catenin pathway. g qRT-PCR was used to determine expression of CTNNB1 after transfecting with sh-FLVCR1-AS1#1. h Subcellular fractionation and FISH were performed to detect the location of FLVCR1-AS1. ^**P < 0.01