Fig. 4

FLVCR1-AS1 competitively binds to miR-381-3p against CTNNB1. a Shared combinable miRNAs was detected by Starbase. b qRT-PCR analysis was conducted to evaluate miR-381-3p expression after knockdown of FLVCR1-AS1. c RT-PCR analysis was conducted to examine the PART1 expression level in BC cell lines and normal cell lines. d Putative miR-381-3p binding site with FLVCR1-AS1 predicted on Starbase. e Luciferase reporter assay performed in HEK-293T cell line confirmed the interaction between miR-381-3p and FLVCR1-AS1. f RNA pull down showed enriched miR-381-3p in biotin-labeled FLVCR1-AS1 wild type group. g The transfection efficiency of miR-381-3p inhibitor was assessed. h Colony formation was performed to determine cell proliferation among sh-Ctrl, sh-FLVCR1-AS1#1 and sh-FLVCR1-AS1#1 + inhibitor. i TUNEL assay was used to examine cell apoptosis among sh-Ctrl, sh-FLVCR1-AS1#1 and sh-FLVCR1-AS1#1 + inhibitor. j, k Transwell assays were used to measure cell migration and invasion among sh-Ctrl, sh-FLVCR1-AS1#1 and sh-FLVCR1-AS1#1 + inhibitor. l Western blot was performed to examine the expression of proteins associated with cell apoptosis and migration. m Potential binding site between miR-381-3p and CTNNB1 predicted on Starbase. n, o Dual luciferase reporter and RIP were performed to verify the interaction. ^**P < 0.01